stereo seq library preparation  (Complete Genomics Inc)

Journal: Horticulture Research

Article Title: Uncovering the genetic architecture of pungency, carotenoids, and flavor in Capsicum chinense via TWAS-mGWAS integration and spatial transcriptomics

doi: 10.1093/hr/uhaf243

Figure Lengend Snippet: Spatially resolved transcriptomic analysis of two contrasting C. chinense accessions, PI 656271 and PI 660973, was performed using Stereo-seq on 5-day post-anthesis (5-dpa) fruits to investigate tissue-specific gene expression patterns. Panel A illustrates the application of spatial transcriptomics to map gene activity within intact fruit tissues. Panel B presents a Uniform Manifold Approximation and Projection (UMAP) plot of spatial transcriptomic spots, where each dot represents an individual spatial location, and colors indicate distinct transcriptional clusters across the fruit sections. Panels C and D display the differential spatial expression of AP2 (APETALA2) transcription factors and PPR (Pentatricopeptide Repeat) genes, respectively, within Modules 1 and 2—gene modules identified as highly spatially correlated and differentially expressed between the two accessions. These spatial expression profiles reveal zone-specific regulatory networks involved in the biosynthesis of capsaicinoids, carotenoids, and volatile metabolites, offering insights into the tissue-level transcriptional control of fruit quality traits in C. chinense .

Article Snippet: The recovered cDNA was used for spatial library preparation following the Stereo-seq Library Preparation Kit (BGI, catalog 111KL114) protocol.

Techniques: Gene Expression, Activity Assay, Expressing, Control